pPV230.13
(Plasmid #14902)

• Depositing Lab: James Lok
• Publication: Li et al Int J Parasitol. 2006 May 31. 36(6):671-9.

Backbone

• Vector backbone: pPD95.81
• Backbone manufacturer: Andrew Fire
• Backbone size w/o insert (bp): 4492
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region
• Alt name: era-1 promoter
• Species: S. stercoralis (nematode)
• Insert Size (bp): 958
• Mutation: Ss era-1 promoter region as a HindIII-PstI fragment insterted at 149-1112, Ss era-1 3' UTR as an EcorI-EagI fragment inserted at 2116-2713
• GenBank ID: AH015511

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: M13

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry