pGBW-m4046182
(Plasmid #149019)

• Purpose: Yeast Expression plasmid for SARS-CoV-2 nsp4
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: SARS-CoV-2 protein Yeast expression constructs, tagged, for protein expression and/or purification (unpublished)

Backbone

• Vector backbone: AmpR; 2u URA3
• Backbone size w/o insert (bp): 4078
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 nsp4
• Species: S. cerevisiae (budding yeast); Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 1499
• Mutation: Saccharomyces cerevisiae recode 1
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: pTEF1
• Tag / Fusion Protein:
  • Cleavable thrombin;3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.