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Addgene

pYL192 (TRV1)
(Plasmid #148968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 148968 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIN19
  • Backbone size w/o insert (bp) 11498
  • Total vector size (bp) 19370
  • Vector type
    Plant vector with TRV RNA1; this vector used with pYL156(TRV RNA2) for virus induced gene silencing (VIGS)
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    We prefer to use DH10B cells
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TRV RNA1
  • Species
    Tobacco Rattle Virus RNA 1
  • Insert Size (bp)
    6791
  • Mutation
    P697S mutation in putative replicase. S239G and S246P mutation in 16kDa protein.
  • GenBank ID
    AF406990

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYL192 (TRV1) was a gift from Savithramma Dinesh-Kumar (Addgene plasmid # 148968 ; http://n2t.net/addgene:148968 ; RRID:Addgene_148968)

  • For your References section:

    Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP. Plant J. 2002 May;30(4):415-29. doi: 10.1046/j.1365-313x.2002.01297.x. 10.1046/j.1365-313x.2002.01297.x PubMed 12028572
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