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pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI
(Plasmid #148949)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 148949 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsGIGYF2_1-718-del280-310
  • Species
    H. sapiens (human)
  • Mutation
    one silent mutation and one deletion of Q1211 compared to the sequence of NCBI: NM_015575.3 or Uniprot.: Q6Y7W6-1
  • Entrez Gene
    GIGYF2 (a.k.a. GYF2, PARK11, PERQ2, PERQ3, TNRC15)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI was a gift from Elisa Izaurralde (Addgene plasmid # 148949 ; http://n2t.net/addgene:148949 ; RRID:Addgene_148949)

  • For your References section:

    Molecular basis for GIGYF-Me31B complex assembly in 4EHP-mediated translational repression. Peter D, Ruscica V, Bawankar P, Weber R, Helms S, Valkov E, Igreja C, Izaurralde E. Genes Dev. 2019 Oct 1;33(19-20):1355-1360. doi: 10.1101/gad.329219.119. Epub 2019 Aug 22. 10.1101/gad.329219.119 PubMed 31439631
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