pAc5.1B-EGFP-DmGIGYF_AH
(Plasmid #148918)

• Purpose: Insect Expression of DmGIGYF
• Depositing Lab: Elisa Izaurralde
• Publication: Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 10.1093/nar/gkz429.

Backbone

• Vector backbone: Unknown
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DmGIGYF
• Species: D. melanogaster (fly)
• Mutation: one silent mutation compared to the sequence given by isoform G NCBI:NM_001169356 or Uniprot.:Q7KQM6
• Entrez Gene: Gyf (a.k.a. Dmel_CG11148, CG11148, Dmel\CG11148, Q7KQM6)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results.

Addgene QC NGS identifies a number of discrepancies in the Ac5 promoter. It is unknown what, if any, impact these may have on plasmid function.