pETM-41P-HsXRN1_1174-1649-Strep_AH
(Plasmid
#148901)
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PurposeBacterial Expression of HsXRN1_1174-1649
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 148901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHsXRN1_1174-1649
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SpeciesH. sapiens (human)
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Entrez GeneXRN1 (a.k.a. SEP1)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sequence given by NCBI: NM_019001.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM-41P-HsXRN1_1174-1649-Strep_AH was a gift from Elisa Izaurralde (Addgene plasmid # 148901 ; http://n2t.net/addgene:148901 ; RRID:Addgene_148901) -
For your References section:
A low-complexity region in human XRN1 directly recruits deadenylation and decapping factors in 5'-3' messenger RNA decay. Chang CT, Muthukumar S, Weber R, Levdansky Y, Chen Y, Bhandari D, Igreja C, Wohlbold L, Valkov E, Izaurralde E. Nucleic Acids Res. 2019 Sep 26;47(17):9282-9295. doi: 10.1093/nar/gkz633. 10.1093/nar/gkz633 PubMed 31340047