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Addgene

pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH
(Plasmid #148897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 148897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsHelz-sgRNA
  • Species
    H. sapiens (human)
  • Entrez Gene
    HELZ (a.k.a. DHRC, DRHC, HUMORF5)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH was a gift from Elisa Izaurralde (Addgene plasmid # 148897 ; http://n2t.net/addgene:148897 ; RRID:Addgene_148897)

  • For your References section:

    HELZ directly interacts with CCR4-NOT and causes decay of bound mRNAs. Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L. Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 10.26508/lsa.201900405. Print 2019 Oct. 10.26508/lsa.201900405 PubMed 31570513
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