IgK-IFN-luc
(Plasmid #14886)

• Purpose: Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
• Depositing Lab: David Baltimore
• Publication: Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IgK-IFN
• Alt name: IFNB1
• Alt name: IgK2‐IFN‐LUC reporter
• Species: H. sapiens (human)
• Insert Size (bp): 80
• Entrez Gene: IFNB1 (a.k.a. IFB, IFF, IFN-beta, IFNB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains two copies of the immunoglobulin kappa light chain kappa-B site (5'-GGGGACTTTCC-3') upstream of the interferon-beta minimal promoter (–55 to +19) (Fujita et al., 1987) driving luciferase expression.