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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRL.sin-18.ppt
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemutant LEF-1/TCF-responsive promoter
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Alt namebeta catenin reporter
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Insert Size (bp)100
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Mutationmutant TCF/LEF binding sites
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Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
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Tag
/ Fusion Protein
- d2 eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
d2-eGFP gene (destabilized, half-life of 2 h; Clontech) was cloned downstream of a mutant LEF-1/TCF-responsive promoter, containing three mutant LEF-1/TCF binding motifs and a TATA box. This cassette was then cloned into a self-inactivating lentiviral vector plasmid.
The author's map is for the wild-type version of this plasmid, Lentiviral-TOP-dGFP-reporter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lentiviral-FOP-dGFP-reporter was a gift from Tannishtha Reya (Addgene plasmid # 14885 ; http://n2t.net/addgene:14885 ; RRID:Addgene_14885) -
For your References section:
A role for Wnt signalling in self-renewal of haematopoietic stem cells. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL. Nature. 2003 May 22. 423(6938):409-14. 10.1038/nature01593 PubMed 12717450