FUGW
(Plasmid #14883)

• Purpose: 3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion
• Depositing Lab: David Baltimore
• Publication: Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Backbone

• Vector backbone: HR'CS-G
• Backbone manufacturer: I. Verma, Salk
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: flap-Ub promoter-GFP-WRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 211 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid pFUGW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap sequence PCR-amplified
from the HIV NLA4.3 genome, the human polyubiquitin promoter-C (gift of L. Thiel, Amgen), the EGFP gene, and the WRE (woodchuck
hepatitis virus posttranscriptional regulatory element) (gift of D. Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.

Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI SmaI KpnI GFP NotI EagI XbaI EcoRI EcoRV HindIII ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI.