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Addgene

DKAR-T/A
(Plasmid #14880)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14880 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D kinase activity reporter
  • Alt name
    CFP-FHA2-peptide-YFP
  • Mutation
    T/A
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The phosphoacceptor threonine was mutated to an alanine to create DKAR-T/A. Substrate sequence: LSRQLAAAVSE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DKAR-T/A was a gift from Alexandra Newton (Addgene plasmid # 14880 ; http://n2t.net/addgene:14880 ; RRID:Addgene_14880)

  • For your References section:

    Calcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. Kunkel MT, Toker A, Tsien RY, Newton AC. J Biol Chem. 2007 Mar 2. 282(9):6733-42. 10.1074/jbc.M608086200 PubMed 17189263
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