pnEA-NpM-HsNot4-GB1His6_AG
(Plasmid #148792)

• Purpose: Bacterial Expression of HsNot4
• Depositing Lab: Elisa Izaurralde
• Publication: Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. doi: 10.1101/gad.320952.118. Epub 2019 Jan 28.

Backbone

• Vector backbone: Unknown
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HsNot4
• Species: H. sapiens (human)
• Mutation: one silent mutation compared to the sequence given by variant 4 NCBI: NM_001190848.1 or Uniprot.: isoform 1 O95628-1
• Entrez Gene: CNOT4 (a.k.a. CLONE243, NOT4, NOT4H)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact before placing your order to request that our lab fully sequence the plasmid.