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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameD kinase activity reporter
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Alt nameCFP-FHA2-peptide-YFP
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Tags
/ Fusion Proteins
- CFP (N terminal on insert)
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DKAR was generated through substitution of a PKD substrate sequence, LSRQLTAAVSE, for the substrate sequence within CKAR.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DKAR was a gift from Alexandra Newton (Addgene plasmid # 14879 ; http://n2t.net/addgene:14879 ; RRID:Addgene_14879) -
For your References section:
Calcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. Kunkel MT, Toker A, Tsien RY, Newton AC. J Biol Chem. 2007 Mar 2. 282(9):6733-42. 10.1074/jbc.M608086200 PubMed 17189263
Map uploaded by the depositor.
