pRSET B-BKAR
(Plasmid
#14877)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSET B
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameB kinase activity reporter
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Alt nameCFP-FHA2-peptide-YFP
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Tags
/ Fusion Proteins
- CFP (N terminal on insert)
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site PvuII (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PKB substrate sequence: RKRDRLGTLGI.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET B-BKAR was a gift from Alexandra Newton (Addgene plasmid # 14877 ; http://n2t.net/addgene:14877 ; RRID:Addgene_14877) -
For your References section:
Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. Kunkel MT, Ni Q, Tsien RY, Zhang J, Newton AC. J Biol Chem. 2005 Feb 18. 280(7):5581-7. 10.1074/jbc.M411534200 PubMed 15583002
Map uploaded by the depositor.
