YFP-DBD
(Plasmid #14874)

• Depositing Lab: Alexandra Newton
• Publication: Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-56.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DAG binding domain
• Alt name: C1b-YFP
• Alt name: PKC beta II
• Species: R. norvegicus (rat)
• Mutation: Y123W
• Entrez Gene: Prkcb (a.k.a. Pkcb, Prkcb1)
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

YFP-tagged DAG binding domain (YFP-DBD) was first constructed by fusion of monomeric YFP to the 5'-end of the C1b domain of PKCbeta. This construct was improved for DAG binding by introducing the Y123W point mutation via QuikChange (Stratagene).