pnYC-CvH-CteIF4E_35-250-del183-202-I87AI91A_AF
(Plasmid
#148722)
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PurposeBacterial Expression of CteIF4E_35-250-del183-202-I87AI91A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 148722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCteIF4E_35-250-del183-202-I87AI91A
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SpeciesChaetomium thermophilum
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Geneart clone of CtEIF4E, codon optimized for Expression in E.coli. (sequence according to Uniprot.: G0SCU4) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pnYC-CvH-CteIF4E_35-250-del183-202-I87AI91A_AF was a gift from Elisa Izaurralde (Addgene plasmid # 148722 ; http://n2t.net/addgene:148722 ; RRID:Addgene_148722) -
For your References section:
Structural motifs in eIF4G and 4E-BPs modulate their binding to eIF4E to regulate translation initiation in yeast. Gruner S, Weber R, Peter D, Chung MY, Igreja C, Valkov E, Izaurralde E. Nucleic Acids Res. 2018 Jul 27;46(13):6893-6908. doi: 10.1093/nar/gky542. 10.1093/nar/gky542 PubMed 30053226