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Addgene

MitoCKAR
(Plasmid #14872)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C kinase activity reporter
  • Alt name
    TOM20-CFP-FHA2-peptide-YFP
  • Tags / Fusion Proteins
    • TOM20(1-33) (N terminal on insert)
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MitoCKAR was generated by fusing in frame sequences encoding the amino-terminal 33 amino acids of TOM 20 to the immediate 5'-end of CKAR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MitoCKAR was a gift from Alexandra Newton (Addgene plasmid # 14872 ; http://n2t.net/addgene:14872 ; RRID:Addgene_14872)

  • For your References section:

    Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. Gallegos LL, Kunkel MT, Newton AC. J Biol Chem. 2006 Oct 13. 281(41):30947-56. 10.1074/jbc.M603741200 PubMed 16901905
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