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Addgene

pnEA-NpM-ScEap1p_80-122_AF
(Plasmid #148707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 148707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ScEap1p_80-122
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    EAP1 (a.k.a. YKL204W)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Geneart clone of ScEAP1, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P36041) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnEA-NpM-ScEap1p_80-122_AF was a gift from Elisa Izaurralde (Addgene plasmid # 148707 ; http://n2t.net/addgene:148707 ; RRID:Addgene_148707)

  • For your References section:

    Structural motifs in eIF4G and 4E-BPs modulate their binding to eIF4E to regulate translation initiation in yeast. Gruner S, Weber R, Peter D, Chung MY, Igreja C, Valkov E, Izaurralde E. Nucleic Acids Res. 2018 Jul 27;46(13):6893-6908. doi: 10.1093/nar/gky542. 10.1093/nar/gky542 PubMed 30053226
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