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Addgene

CYPHR
(Plasmid #14864)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyan/yellow PH domain reporter
  • Alt name
    CFP-PHdelta-YFP
  • Alt name
    PLC delta I
  • Entrez Gene
    Plcd1
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CFP and YFP fused to the PH domain of PLCdelta. CYPHR was generated by PCR of CFP from HindIII to KpnI, the PH domain of murine PLCdelta1 (aa 10–140) from KpnI to XhoI, and mYFP from XhoI to XbaI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CYPHR was a gift from Alexandra Newton (Addgene plasmid # 14864 ; http://n2t.net/addgene:14864 ; RRID:Addgene_14864)

  • For your References section:

    A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. Violin JD, Zhang J, Tsien RY, Newton AC. J Cell Biol. 2003 Jun 9. 161(5):899-909. 10.1083/jcb.200302125 PubMed 12782683
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