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Addgene

pHR' CMV GFP
(Plasmid #14858)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14858 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR'
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV-GFP
  • Insert Size (bp)
    1340

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HIV vector with CMV promoter. Constructed by inserting the CMV-GFP fragment from pKS-CMV-GFP into the same sites of pHR'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR' CMV GFP was a gift from Inder Verma (Addgene plasmid # 14858 ; http://n2t.net/addgene:14858 ; RRID:Addgene_14858)

  • For your References section:

    Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector. Miyoshi H, Takahashi M, Gage FH, Verma IM. Proc Natl Acad Sci U S A. 1997 Sep 16. 94(19):10319-23. 10.1073/pnas.94.19.10319 PubMed 9294208
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