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pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE
(Plasmid #148565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 148565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hs4EHP_52-234opt-R103LE149L
  • Species
    H. sapiens (human)
  • Entrez Gene
    EIF4E2 (a.k.a. 4E-LP, 4EHP, EIF4EL3, IF4e, h4EHP)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Geneart clone of Hs4EHP codon optimized for expression in E.coli. sequence based on the sequence given by NM_008446.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE was a gift from Elisa Izaurralde (Addgene plasmid # 148565 ; http://n2t.net/addgene:148565 ; RRID:Addgene_148565)

  • For your References section:

    GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression. Peter D, Weber R, Sandmeir F, Wohlbold L, Helms S, Bawankar P, Valkov E, Igreja C, Izaurralde E. Genes Dev. 2017 Jun 1;31(11):1147-1161. doi: 10.1101/gad.299420.117. Epub 2017 Jul 11. 10.1101/gad.299420.117 PubMed 28698298
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