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Addgene

Lox-stop-Lox p53 R270H Notes

6.01.07 Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
Lox-stop-Lox p53 R270H with the following enzyme: Not I

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of 4 DNA preps from 4 individual small colonies containing the plasmid with the following enzyme: NotI

We expected the following band sizes for this digest: ~20kb. We see the expected pattern.