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Addgene

pJRL2 PHO5pr VYFP (EB#1547)
(Plasmid #14840)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14840 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJRL2
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHO5 promoter
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PHO5 (a.k.a. YBR093C)
  • Tag / Fusion Protein
    • VYFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.

The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJRL2 PHO5pr VYFP (EB#1547) was a gift from Erin O'Shea (Addgene plasmid # 14840 ; http://n2t.net/addgene:14840 ; RRID:Addgene_14840)
  • For your References section:

    Control of stochasticity in eukaryotic gene expression. Raser JM, O'Shea EK. Science. 2004 Jun 18. 304(5678):1811-4. 10.1126/science.1098641 PubMed 15166317