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pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB
(Plasmid #148372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 148372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Dm4E-T-F41DW42D
  • Species
    D. melanogaster (fly)
  • Mutation
    5 non silent mutation L127S, E493G, H628R, N659S, S947G and 4 silent mutations compared to the sequence from CG32016 isoform C. NCBI:NM_166798.1.
  • Entrez Gene
    4E-T (a.k.a. Dmel_CG32016, CG11097, CG11232, CG32016, Dm4E-T, Dmel\CG32016)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB was a gift from Elisa Izaurralde (Addgene plasmid # 148372 ; http://n2t.net/addgene:148372 ; RRID:Addgene_148372)

  • For your References section:

    Molecular architecture of 4E-BP translational inhibitors bound to eIF4E. Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, Weichenrieder O, Izaurralde E. Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19. 10.1016/j.molcel.2015.01.017 PubMed 25702871
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