pBabe Flag Smad6
(Plasmid #14835)

• Depositing Lab: Rik Derynck
• Publication: Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Backbone

• Vector backbone: pBabe puro 3
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smad6
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• Entrez Gene: Smad6 (a.k.a. Madh6, b2b390Clo)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBabe 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Masao Takase, K. Miyazono lab, Dept of Biochemistry, Cancer Institute, Tokyo, Japan
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See Author's map for more information.