pAc5.1B-EGFP-DmMextli_577-620_AB
(Plasmid
#148337)
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PurposeInsect Expression of DmMextli_577-520
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 148337 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDmMextli_577-520
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SpeciesD. melanogaster (fly)
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Entrez Genemxt (a.k.a. Dmel_CG2950, BcDNA:GH06479, CG2950, Dmel\CG2950, cg2950)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sequence given by NCBI:NM_164598. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1B-EGFP-DmMextli_577-620_AB was a gift from Elisa Izaurralde (Addgene plasmid # 148337 ; http://n2t.net/addgene:148337 ; RRID:Addgene_148337) -
For your References section:
Mextli proteins use both canonical bipartite and novel tripartite binding modes to form eIF4E complexes that display differential sensitivity to 4E-BP regulation. Peter D, Weber R, Kone C, Chung MY, Ebertsch L, Truffault V, Weichenrieder O, Igreja C, Izaurralde E. Genes Dev. 2015 Sep 1;29(17):1835-49. doi: 10.1101/gad.269068.115. Epub 2015 Aug 20. 10.1101/gad.269068.115 PubMed 26294658