pRK5 TGF beta type I receptor (T202D) Flag
(Plasmid #14833)

• Depositing Lab: Rik Derynck
• Publication: Feng et al J Biol Chem. 1996 May 31. 271(22):13123-9.

Backbone

• Vector backbone: pRK5 modified with flag
• Backbone manufacturer: Genentech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGF beta type I receptor
• Alt name: TGFb type I receptor
• Alt name: Tgfbr1
• Alt name: ALK5
• Species: R. norvegicus (rat)
• Mutation: Threonine 202 mutated to aspartate. Constitutively active mutant.
• Entrez Gene: Tgfbr1 (a.k.a. Alk5, Skr4, Tgfr-1, tbetaR-I)
• Tag / Fusion Protein:
  • flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See Author's map for more information.

A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.