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Addgene

pRK Smad3N Flag
(Plasmid #14827)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Genentech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3 N
  • Alt name
    Smad3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    440
  • Mutation
    amino acids 1-146
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Author's map for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK Smad3N Flag was a gift from Rik Derynck (Addgene plasmid # 14827 ; http://n2t.net/addgene:14827 ; RRID:Addgene_14827)

  • For your References section:

    The tumor suppressor Smad4/DPC 4 as a central mediator of Smad function. Zhang Y, Musci T, Derynck R. Curr Biol. 1997 Apr 1. 7(4):270-6. 10.1016/S0960-9822(06)00123-0 PubMed 9094310
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