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Addgene

XFIVP Smad3
(Plasmid #14826)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    XFIVP / pRK5
  • Backbone manufacturer
    Genentech
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
  • Tag / Fusion Protein
    • VP16 activation domain (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length Smad3 fused to VP16 activation domain

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XFIVP Smad3 was a gift from Rik Derynck (Addgene plasmid # 14826 ; http://n2t.net/addgene:14826 ; RRID:Addgene_14826)

  • For your References section:

    The tumor suppressor Smad4/DPC4 and transcriptional adaptor CBP/p300 are coactivators for smad3 in TGF-beta-induced transcriptional activation. Feng XH, Zhang Y, Wu RY, Derynck R. Genes Dev. 1998 Jul 15. 12(14):2153-63. 10.1101/gad.12.14.2153 PubMed 9679060
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