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Addgene

pRD183 (Roc2)
(Plasmid #14824)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T7 IVT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Roc2
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    400
  • Entrez Gene
    Roc2 (a.k.a. BcDNA:RE61847, CG8998, Dmel\CG8998, dRoc2, Roc)
  • Tag / Fusion Protein
    • Flag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRD183 (Roc2) was a gift from Robert Duronio (Addgene plasmid # 14824 ; http://n2t.net/addgene:14824 ; RRID:Addgene_14824)
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