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Addgene

pRD119 (PCNA GFP)
(Plasmid #14822)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14822 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Backbone manufacturer
    na
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCNA-GFP
  • Alt name
    PCNA
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    PCNA (a.k.a. Dmel_CG9193, 53/13, CG9193, DmPCNA, DmPCNA1, Dmel\CG9193, MUS209, Mus209, PCNA/mus209, PCNA1, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, mus 209, mus209, pcna)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCNA GFP fusion (glow); Asp718-NotI fragment from pRD113 cloned into pCasper4IN; GFP fused in-frame using 5' blunt NheI and 3' XbaI (replaces PCNA coding to STOP [XbaI] but has PCNA 3'; GFP from pNEGFPX.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRD119 (PCNA GFP) was a gift from Robert Duronio (Addgene plasmid # 14822 ; http://n2t.net/addgene:14822 ; RRID:Addgene_14822)

  • For your References section:

    The contribution of E2F-regulated transcription to Drosophila PCNA gene function. Thacker SA, Bonnette PC, Duronio RJ. Curr Biol. 2003 Jan 8. 13(1):53-8. 10.1016/S0960-9822(02)01400-8 PubMed 12526745
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