pRD65 (dDP)
(Plasmid
#14821)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonena
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Backbone manufacturerna
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDP
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SpeciesD. melanogaster (fly)
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Entrez GeneDp (a.k.a. Dmel_CG4654, 49Fk, CG4654, DDP1, DP, DP1, DmDP, Dmel\CG4654, Dp1, TFPD2, dDP, dDP1, dDp, dp, l(2)49Fk, l(2)vr10, vr10)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full length dDP cDNA; 5' end from Nick Brown library with pNB5 and BDO47 primers; cloned HindIII-Nhe1 in pRD63.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRD65 (dDP) was a gift from Robert Duronio (Addgene plasmid # 14821 ; http://n2t.net/addgene:14821 ; RRID:Addgene_14821)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/29/91/a9d26ea4-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)