Syn2DpcDNA4/TO
(Plasmid
#14818)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCDNA4/TO/myc-His
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5150
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSyntaxin2D
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Alt nameSTX2D
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Alt nameEpim
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SpeciesR. norvegicus (rat)
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Insert Size (bp)750
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Entrez GeneStx2 (a.k.a. Epim)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer CMVF
- 3′ sequencing primer pcDNA3.1/BGH reverse primier (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Syn2DpcDNA4/TO was a gift from Thomas Weimbs (Addgene plasmid # 14818 ; http://n2t.net/addgene:14818 ; RRID:Addgene_14818) -
For your References section:
Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells. Low SH, Li X, Miura M, Kudo N, Quinones B, Weimbs T. Dev Cell. 2003 May . 4(5):753-9. 10.1016/S1534-5807(03)00122-9 PubMed 12737809
