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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z
(Plasmid #148162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 148162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Dm4E-T_1-511-YLLLtoAAAA
  • Alt name
    4E-T
  • Species
    D. melanogaster (fly)
  • Mutation
    two non silent mutations L127S, annotated as EST, and P329R compared to CG32016 isoform C. NCBI:NM_166798.1
  • Entrez Gene
    4E-T (a.k.a. Dmel_CG32016, CG11097, CG11232, CG32016, Dm4E-T, Dmel\CG32016)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z was a gift from Elisa Izaurralde (Addgene plasmid # 148162 ; http://n2t.net/addgene:148162 ; RRID:Addgene_148162)

  • For your References section:

    4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation. Igreja C, Peter D, Weiler C, Izaurralde E. Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790. 10.1038/ncomms5790 PubMed 25179781
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