pT7-V5-SBP-C1-Hs4EBP3_Z
(Plasmid
#148151)
-
PurposeMammalian Expression of Hs4EBP3
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 148151 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneUnknown
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHs4EBP3
-
SpeciesH. sapiens (human)
-
Entrez GeneEIF4EBP3 (a.k.a. 4E-BP3, 4EBP3)
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sequence extracted fromNCBI:NM_003732.2 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pT7-V5-SBP-C1-Hs4EBP3_Z was a gift from Elisa Izaurralde (Addgene plasmid # 148151 ; http://n2t.net/addgene:148151 ; RRID:Addgene_148151) -
For your References section:
The Structures of eIF4E-eIF4G Complexes Reveal an Extended Interface to Regulate Translation Initiation. Gruner S, Peter D, Weber R, Wohlbold L, Chung MY, Weichenrieder O, Valkov E, Igreja C, Izaurralde E. Mol Cell. 2016 Nov 3;64(3):467-479. doi: 10.1016/j.molcel.2016.09.020. Epub 2016 Oct 20. 10.1016/j.molcel.2016.09.020 PubMed 27773676