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Addgene

pGex-3x Flag MKK3
(Plasmid #14814)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGex 3x
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4952
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MKK3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1741
  • Entrez Gene
    MAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGex 5'
  • 3′ sequencing primer pGex 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGex-3x Flag MKK3 was a gift from Roger Davis (Addgene plasmid # 14814 ; http://n2t.net/addgene:14814 ; RRID:Addgene_14814)

  • For your References section:

    Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms. Derijard B, Raingeaud J, Barrett T, Wu IH, Han J, Ulevitch RJ, Davis RJ. Science. 1995 Feb 3. 267(5198):682-5. 10.1126/science.7839144 PubMed 7839144
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