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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMKK4(glu)
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Alt nameMKK4
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SpeciesH. sapiens (human)
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Insert Size (bp)1151
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MutationSer-220Glu Thr-224Glu
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Entrez GeneMAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 Flag MKK4(glu) was a gift from Roger Davis (Addgene plasmid # 14813 ; http://n2t.net/addgene:14813 ; RRID:Addgene_14813) -
For your References section:
Integration of MAP kinase signal transduction pathways at the serum response element. Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ. Science. 1995 Jul 21. 269(5222):403-7. 10.1126/science.7618106 PubMed 7618106