pPERPlucFL
(Plasmid
#14811)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4800
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Vector typeBacterial Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePERP
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SpeciesM. musculus (mouse)
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Insert Size (bp)8000
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Entrez GenePerp (a.k.a. 1110017A08Rik, KCP1, KRTCAP1, PIGPC1, THW)
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Tag
/ Fusion Protein
- Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The pPERPlucFL vector was constructed by insertion of a splice acceptor upstream of the luciferase cassette in pGL3Basic and insertion of a BamHI Perp genomic fragment (from -3850 to +4150) into the polylinker region.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPERPlucFL was a gift from Tyler Jacks (Addgene plasmid # 14811 ; http://n2t.net/addgene:14811 ; RRID:Addgene_14811) -
For your References section:
Multiple response elements and differential p53 binding control Perp expression during apoptosis. Reczek EE, Flores ER, Tsay AS, Attardi LD, Jacks T. Mol Cancer Res. 2003 Dec . 1(14):1048-57. PubMed 14707288