pPERPluc1
(Plasmid #14810)

• Depositing Lab: Tyler Jacks
• Publication: Reczek et al Mol Cancer Res. 2003 Dec . 1(14):1048-57.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4800
• Vector type: Bacterial Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PERP promoter
• Alt name: PERP
• Species: M. musculus (mouse)
• Insert Size (bp): 4000
• Entrez Gene: Perp (a.k.a. 1110017A08Rik, KCP1, KRTCAP1, PIGPC1, THW)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pPERPluc1 reporter was constructed by site-directed mutagenesis of the initial ATG of the PERP coding region (to create an NcoI site) using the Quick Change Site Directed mutagenesis kit (Stratagene), followed by joining of the 4-kb PERP promoter region to the initial ATG of luciferase in the pGL3Basic vector (Promega).