pSico Spry-2
(Plasmid
#14809)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSico
- Backbone size w/o insert (bp) 7566
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Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSprouty-2 shRNA
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Alt nameSpry2
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SpeciesM. musculus (mouse)
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Entrez GeneSpry2 (a.k.a. sprouty2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence of the Spry-2 shRNA is 5'-GCCGG GTTGT CGTTG TAAA-3' and corresponds to nucleotides 1150–1168 of mSpry-2.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSico Spry-2 was a gift from Tyler Jacks (Addgene plasmid # 14809 ; http://n2t.net/addgene:14809 ; RRID:Addgene_14809) -
For your References section:
Sprouty-2 regulates oncogenic K-ras in lung development and tumorigenesis. Shaw AT, Meissner A, Dowdle JA, Crowley D, Magendantz M, Ouyang C, Parisi T, Rajagopal J, Blank LJ, Bronson RT, Stone JR, Tuveson DA, Jaenisch R, Jacks T. Genes Dev. 2007 Mar 15. 21(6):694-707. 10.1101/gad.1526207 PubMed 17369402