pHes1-GFPd2
(Plasmid #14808)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104

Backbone

• Vector backbone: pd2EGFP-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4244
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hes1 promoter
• Insert Size (bp): 1121
• Tag / Fusion Protein:
  • GFPd2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Hes1 promoter is specific for progenitor cells in the developing retina. This promoter is also weakly active in Muller glia in the differentiated retina.