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Addgene

pBabe-puro/HA-FLAG-RBP2 H483A
(Plasmid #14803)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Retinoblastoma Binding Protein 2
  • Alt name
    RBP2
  • Alt name
    JARID1A
  • Alt name
    KDM5A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4900
  • Mutation
    changed His 483 to Ala
  • Entrez Gene
    KDM5A (a.k.a. RBBP-2, RBBP2, RBP2)
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid encodes isoform 2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro/HA-FLAG-RBP2 H483A was a gift from William Kaelin (Addgene plasmid # 14803 ; http://n2t.net/addgene:14803 ; RRID:Addgene_14803)

  • For your References section:

    The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG. Cell. 2007 Mar 9. 128(5):889-900. 10.1016/j.cell.2007.02.013 PubMed 17320163
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