pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537SQ1658A_W
(Plasmid
#147869)
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PurposeInsect Expression of DmNot1_1467-1717-K1537SQ1658A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 147869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDmNot1_1467-1717-K1537SQ1658A
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SpeciesD. melanogaster (fly)
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Entrez GeneNot1 (a.k.a. Dmel_CG34407, CG1874, CG1884, CG34407, Dmel\CG34407, Dmel_CG1874, Dmel_CG1884, NOT, NOT1, anon-EST:Liang-1.29, anon-WO0118547.221, clone 1.29, not1)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sequence given by NCBI: NM_001103772.2. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537SQ1658A_W was a gift from Elisa Izaurralde (Addgene plasmid # 147869 ; http://n2t.net/addgene:147869 ; RRID:Addgene_147869) -
For your References section:
A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E. Mol Cell. 2014 Jun 5;54(5):737-50. doi: 10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24. 10.1016/j.molcel.2014.03.034 PubMed 24768540