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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV-neo
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Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 6500
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelet-7g
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
miRNA of the following mature sequence, with approximately 500 basepairs of flanking sequence, were cloned into MSCV-neo: let-7g: 5’-UGAGG UAGUA GUUUG UACAG U-3’.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV let-7g was a gift from Tyler Jacks (Addgene plasmid # 14784 ; http://n2t.net/addgene:14784 ; RRID:Addgene_14784) -
For your References section:
Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 Apr 1. ():. 10.1038/ng2003 PubMed 17401365