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pnYC-NpM-HsSMG7opt_732-1091_V
(Plasmid #147769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 147769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HsSMG7opt_732-1091
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMG7 (a.k.a. C1orf16, EST1C, SGA56M)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HsSmg7 codon optimized DNA for expresssion in E. coli (GeneArt) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnYC-NpM-HsSMG7opt_732-1091_V was a gift from Elisa Izaurralde (Addgene plasmid # 147769 ; http://n2t.net/addgene:147769 ; RRID:Addgene_147769)

  • For your References section:

    The SMG5-SMG7 heterodimer directly recruits the CCR4-NOT deadenylase complex to mRNAs containing nonsense codons via interaction with POP2. Loh B, Jonas S, Izaurralde E. Genes Dev. 2013 Oct 1;27(19):2125-38. doi: 10.1101/gad.226951.113. 10.1101/gad.226951.113 PubMed 24115769
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