pSuper mouse DGCR8-2
(Plasmid
#14773)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUPER.retro.puro
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Backbone manufacturerOligoEngine
- Backbone size w/o insert (bp) 6350
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDGCR8 shRNA
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Alt nameDGCR8
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SpeciesM. musculus (mouse)
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Entrez GeneDgcr8 (a.k.a. D16H22S1742E, D16H22S788E, D16Wis2, Gy1, N41, Vo59c07, mir-1306)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
shRNA sequence: GATCCCCCTC TCAATGTGAC TGGATATTCA AGAGATATCC AGTCACATTG AGAGTTTTTG GAAC
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSuper mouse DGCR8-2 was a gift from Tyler Jacks (Addgene plasmid # 14773 ; http://n2t.net/addgene:14773 ; RRID:Addgene_14773) -
For your References section:
Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 Apr 1. ():. 10.1038/ng2003 PubMed 17401365