pAc5.1B-EGFP-Dm4E-T-L36AL39A_T
(Plasmid #147604)

• Purpose: Insect Expression of Dm4E-T-L36AL39A
• Depositing Lab: Elisa Izaurralde
• Publication: Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.

Backbone

• Vector backbone: Unknown
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Dm4E-T-L36AL39A
• Species: D. melanogaster (fly)
• Mutation: Two non silent mutations (H628R and N659S) compared to the sequence given by NCBI: NM_166798.1.
• Entrez Gene: 4E-T (a.k.a. Dmel_CG32016, CG11097, CG11232, CG32016, Dm4E-T, Dmel\CG32016)

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pAc5.1B-EGFP-Dm4E-T-L36AL39A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ORF extracted from CG32016 isoform C. NCBI:NM_166798.1, one variation L127S annotated as EST. Please note that the Addgene verified sequence may be slightly different compared to the depositor's reference sequences. These differences do not affect plasmid function. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact before placing your order to request that our lab fully sequence the plasmid.