pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T
(Plasmid
#147588)
-
PurposeInsect Expression of DmeIF4Ga_578-650-CG32016-E
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 147588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneUnknown
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameDmeIF4Ga_578-650-CG32016-E
-
SpeciesD. melanogaster (fly)
-
MutationCG32016-E 5 silent and three no silent mutations H117R, N148S and P315H compared to the sequence given by NCBI:NM_166800.1
-
Entrez GeneeIF4G1 (a.k.a. Dmel_CG10811, CG10811, Dmel\CG10811, EIF4G, Eif4G, deIF4G, eIF-4G, eIF4G, eIF4g, p200)
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DmEIF4G ORF extracted from NCBI: NM_079901.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T was a gift from Elisa Izaurralde (Addgene plasmid # 147588 ; http://n2t.net/addgene:147588 ; RRID:Addgene_147588) -
For your References section:
4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation. Igreja C, Peter D, Weiler C, Izaurralde E. Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790. 10.1038/ncomms5790 PubMed 25179781