pCAG-mir30
(Plasmid #14758)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4779
• Vector type: Mammalian Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mir30 cassette
• Species: H. sapiens (human)
• Insert Size (bp): 205

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The mir30 cassette was amplified by PCR using pSM2 (Open Biosystems) as a template.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab (http://hannonlab.cshl.edu/GH_protocols.html )(Paddison et al. Nat. Methods 1, 163-167 (2004)).

Note that there are some discrepancies between the Addgene quality control sequence and the assembled sequence from the depositor. These differences are not known to affect function.