HA PKB T308D S473D pcDNA3
(Plasmid #14751)

• Depositing Lab: Jim Woodgett
• Publication: Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKB DD
• Alt name: Protein Kinase B alpha
• Alt name: Akt1
• Species: B. taurus (bovine)
• Insert Size (bp): 1500
• Mutation: T308D S473D. Constitutively active mutant.
• Entrez Gene: AKT1 (a.k.a. BOS_20453)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

KpnI(1934) and NheI(2429) represent T308->D and S473->D mutations respectively